The primitive growth factor NME7AB induces mitochondrially active naïve-like pluripotent stem cells

Carla O'Reilly; Qian Qi; Jennifer L Peters; Yong Cheng; Sang-Oh Yoon; Min-Joon Han

doi:10.1016/j.bbrep.2019.100656

The primitive growth factor NME7_AB induces mitochondrially active naïve-like pluripotent stem cells

Biochem Biophys Rep. 2019 Aug 20:20:100656. doi: 10.1016/j.bbrep.2019.100656. eCollection 2019 Dec.

Authors

Carla O'Reilly¹, Qian Qi¹, Jennifer L Peters², Yong Cheng¹, Sang-Oh Yoon³, Min-Joon Han¹

Affiliations

¹ Department of Hematology, St. Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN, 38105, United States.
² Cell and Tissue Imaging Center, Light Microscopy Shared Resource, St. Jude Children's Research Hospital, 262 Danny Thomas Place Memphis, TN, 38105, United States.
³ University of Illinois College of Medicine, Peoria, IL, 61605, United States.

Abstract

Naïve pluripotent stem cells (PSCs) display a distinctive phenotype when compared to their "primed" counterparts, including, but not limited to, increased potency to differentiate and more robust mitochondrial respiration. The cultivation and maintenance of naïve PSCs have been notoriously challenging, requiring the use of complex cytokine cocktails. NME7_AB is a newly discovered embryonic stem cell growth factor that is expressed exclusively in the first few days of human blastocyst development. It has been previously reported that growing primed induced PSCs (iPSCs) in bFGF-depleted medium with NME7_AB as the only added growth factor facilitates the regression of these cells to their naïve state. Here, we confirm this regression by demonstrating the reactivation of mitochondrial function in the induced naïve-like PSCs and increased ATP production in these cells, as compared to that in primed iPSCs.

Keywords: ERK/Smad signaling; Mitochondria; Naïve stem cell; Oxidative phosphorylation.

Abstract

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