Abstract
Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis Proteins / metabolism*
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Carcinoma, Squamous Cell / drug therapy
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Carcinoma, Squamous Cell / metabolism*
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Carcinoma, Squamous Cell / pathology
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Cholesterol / metabolism
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Cytoplasm / metabolism
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HEK293 Cells
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Humans
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Indoleacetic Acids / pharmacology*
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Plant Growth Regulators / pharmacology
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Proteolysis / drug effects*
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Receptors, Cell Surface / metabolism*
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Skin Neoplasms / drug therapy
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Skin Neoplasms / metabolism*
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Skin Neoplasms / pathology
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Transcription Factors / metabolism*
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Tumor Cells, Cultured
Substances
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AFB2 protein, Arabidopsis
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Arabidopsis Proteins
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IAA7 protein, Arabidopsis
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Indoleacetic Acids
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Plant Growth Regulators
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Receptors, Cell Surface
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Transcription Factors
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Cholesterol