Preparation and characterization of monoclonal antibodies against a form of hamster liver cytochrome P-450 highly specific to aflatoxin B1

Biochem Biophys Res Commun. 1988 May 31;153(1):441-7. doi: 10.1016/s0006-291x(88)81244-0.

Abstract

Monoclonal antibodies were prepared against a form of cytochrome P-450 (designated as cytochrome P-450-I) purified from 3-methylcholanthrene-treated hamster livers which is highly specific to aflatoxin B1. The cytochrome P-450-I was detected in ELISA and Western blots in liver microsomes from 3-methylcholanthrene-treated hamsters and also from non-treated and phenobarbital-treated hamsters in smaller amounts. However, none of the liver microsomes from 3-methylcholanthrene-treated rat, rabbit, guinea pig and Suncus murinus contained the cytochrome P-450-I. These results suggest that cytochrome P-450-I is specific to hamster and is induced mainly by 3-methylcholanthrene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aflatoxin B1
  • Aflatoxins / metabolism*
  • Animals
  • Antibodies, Monoclonal*
  • Cricetinae
  • Cytochrome P-450 Enzyme System / immunology*
  • Cytochrome P-450 Enzyme System / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Mice
  • Mice, Inbred BALB C
  • Microsomes, Liver / enzymology*
  • Rats

Substances

  • Aflatoxins
  • Antibodies, Monoclonal
  • Cytochrome P-450 Enzyme System
  • Aflatoxin B1