Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure

Qingshan Shen; Chunhui Zhang; Wei Jia; Xiaojie Qin; Zhenkun Cui; Haizhen Mo; Aurore Richel

doi:10.1016/j.carbpol.2019.115015

Co-production of chondroitin sulfate and peptide from liquefied chicken sternal cartilage by hot-pressure

Carbohydr Polym. 2019 Oct 15:222:115015. doi: 10.1016/j.carbpol.2019.115015. Epub 2019 Jun 21.

Authors

Qingshan Shen¹, Chunhui Zhang², Wei Jia³, Xiaojie Qin³, Zhenkun Cui⁴, Haizhen Mo⁴, Aurore Richel⁵

Affiliations

¹ Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; Department of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China; University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.
² Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: dr_zch@163.com.
³ Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
⁴ Department of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.
⁵ University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.

PMID: 31320074
DOI: 10.1016/j.carbpol.2019.115015

Abstract

Co-production of chondroitin sulfate (CS) and peptides was realized from the liquid fraction of chicken sternal cartilage subjected to hot-pressure (HP) by membrane combination separation technology. Cartilage was liquefied via the HP treatment at 110 °C (0.07 MPa) and 120 °C (0.1 MPa) for 0.5 - 2.5 h, respectively. The optimized co-production procedure was as follows: enzymolysis temperature, 61.2 °C; the enzyme ratio of trypsin and papain, 1.3:1 (W/W); enzymolysis time ratio, 2:2 (h/h), under which the highest yields of CS and peptides were 18.85% and 67.99%, and the recoveries were 93.63% and 92.69%. The average molecular weight of CS sample was 67.79 kDa. CS sample was confirmed using agarose-gel electrophoresis, and the structure was analyzed by Fourier transform infrared spectroscopy, chromatography and nuclear magnetic resonance. Taken together, HP can be as a pretreatment method to liquefy cartilage for the industrial co-production of CS and peptides with eco-friendly.

Keywords: Chondroitin sulfate; Co-production; Hot-pressure; Liquefaction; Peptides.