Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay

Xiaoying Gao; Xiao Shu; Yinuo Song; Jie Cao; Minsong Gao; Fengqin Wang; Yizhen Wang; Jing Zhi Sun; Jianzhao Liu; Ben Zhong Tang

doi:10.1039/c9cc03923f

Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay

Chem Commun (Camb). 2019 Jul 11;55(57):8321-8324. doi: 10.1039/c9cc03923f.

Authors

Affiliations

¹ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, China. liujz@zju.edu.cn.
² College of Animal Sciences, Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, China.
³ Department of Chemistry, Division of Biomedical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. tangbenz@ust.hk and SCUT-HKUST Joint Research Laboratory, Guangdong Innovative Research Team, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510640, China.

PMID: 31257377
DOI: 10.1039/c9cc03923f

Abstract

Here we report a combined fluorescence and mass spectrometry assay which is capable of stably visualizing and quantifying cellular nucleoside-labeled RNA production and degradation. The fluorescence and mass spectrometry signals from cellular labeled RNAs show a linear correlation. This simple and robust assay benefits the biological community to study RNA metabolism.