[Anti-CD3 monoclonal antibodies. Characterization and function]

Pathol Biol (Paris). 1987 Dec;35(10):1285-91.
[Article in French]

Abstract

Four anti-T lymphocyte monoclonal antibodies were produced by the hybridoma technique. Percentages of miscellaneous labelled cell suspensions, as profiles of fluorescent histograms, showed a reactivity of these 4 reagents quite comparable to that observed with anti-CD3 monoclonal antibodies. Labelling summation studies, cell-sorting studies and blocking experiments gave similar results as gave other anti-CD3 reagents. Biochemical study and functional tests: modulation effects and mitogenic properties, argued for the belonging of these 4 antibodies to the CD3 cluster. The actual data concerning the CD3 molecule are briefly resumed to underline the interest of such antibodies in the understanding of T-lymphocyte activation mechanisms during the immune response.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / physiology
  • Antibody Specificity
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • Antigens, Surface / immunology
  • Flow Cytometry
  • Fluorescein-5-isothiocyanate
  • Fluoresceins
  • Fluorescent Dyes
  • Hybridomas / immunology
  • Leukemia, Lymphoid / immunology
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Mitosis
  • T-Lymphocytes / immunology*
  • Thiocyanates

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • Fluoresceins
  • Fluorescent Dyes
  • Thiocyanates
  • Fluorescein-5-isothiocyanate