Abstract
Geobacillus thermoglucosidans DSM2542 is an industrially important microbe, however the complex nutritional requirements of Geobacilli confound metabolic engineering efforts. Previous studies have utilised semi-defined media recipes that contain complex, undefined, biologically derived nutrients which have unknown ingredients that cannot be quantified during metabolic profiling. This study used design of experiments to investigate how individual nutrients and interactions between these nutrients contribute to growth. A mathematically derived defined medium has been formulated that has been shown to robustly support growth of G. thermoglucosidans in two different environmental conditions (96-well plate and shake flask) and with a variety of lignocellulose-based carbohydrates. This enabled the catabolism of industrially relevant carbohydrates to be investigated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Culture Media / metabolism*
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Geobacillus / growth & development*
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Geobacillus / metabolism*
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Metabolome / physiology*
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Metabolomics / methods
Grants and funding
This work was supported by a research grant from Shell Global Solutions BV to JL. JR was supported directly by The University of Exeter, Exeter, UK. Shell Global Solutions BV (JL-07721/14 TO JL) directed the choice of the microbial chassis used in this investigation. KZ and DAP are employed by Shell Technology Centre Houston (STCH) and DAP is an Honorary Research Fellow at the University of Exeter. KZ and DAP were involved in some aspects of the experimental design and analysis of the results but had no influence regarding the decision to publish and the data presented. STCH provided support in the form of salaries for KZ and DAP but did not influence the experimental design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section.