Self-administration of benzodiazepine and cocaine combinations by male and female rhesus monkeys in a choice procedure: role of α1 subunit-containing GABAA receptors

Psychopharmacology (Berl). 2019 Nov;236(11):3271-3279. doi: 10.1007/s00213-019-05286-0. Epub 2019 Jun 10.

Abstract

Rationale: Compounds lacking efficacy at the α1 subunit-containing GABAA (α1GABAA) receptor appear to have reduced abuse potential compared with those having measurable efficacy at this receptor, though their self-administration in nonhuman primates is dependent upon past drug experience.

Objectives: We used a drug vs. drug choice procedure to evaluate the hypothesis that L-838,417, a compound lacking efficacy at αGABAA receptors, would not enhance cocaine choice in monkeys trained to self-administer cocaine. We also hypothesized that zolpidem, a compound with preferential modulation of ⍺1GABAA receptors and midazolam, a nonselective benzodiazepine, would enhance cocaine choice in this procedure.

Methods: One female and three male rhesus monkeys chose between cocaine alone (0.1 mg/kg/injection) vs. the same dose of cocaine combined with midazolam (0.003-0.1 mg/kg/injection), zolpidem (0.003-0.3 mg/kg/injection), or L-838-417 (0.01-0.1 mg/kg/injection). In addition, we evaluated choice between saline and L-838,417 at select doses to determine whether L-838,417 would function as a reinforcer on its own.

Results: Consistent with our hypotheses, midazolam- and zolpidem-cocaine mixtures were chosen over cocaine alone at sufficiently high doses. However, L-838,417-cocaine mixtures also were chosen over cocaine alone in three of four subjects with at least one dose. When available alone vs. saline, L-838,417 did not function as a reinforcer in any subject.

Conclusion: Compounds that lack efficacy at α1GABAA receptors may have low abuse potential compared to classic benzodiazepines, but self-administration of these compounds is context-dependent.

Keywords: Benzodiazepine; Choice; Cocaine; Rhesus monkey; Self-administration.

MeSH terms

  • Animals
  • Benzodiazepines / administration & dosage*
  • Choice Behavior / drug effects*
  • Choice Behavior / physiology*
  • Cocaine / administration & dosage*
  • Dopamine Uptake Inhibitors / administration & dosage*
  • Dose-Response Relationship, Drug
  • Drug Combinations
  • Female
  • Fluorobenzenes / administration & dosage
  • Macaca mulatta
  • Male
  • Receptors, GABA-A / physiology*
  • Self Administration
  • Triazoles / administration & dosage

Substances

  • Dopamine Uptake Inhibitors
  • Drug Combinations
  • Fluorobenzenes
  • GABRA1 protein, human
  • Receptors, GABA-A
  • Triazoles
  • Benzodiazepines
  • L 838,417
  • Cocaine