Consistent production of chlorine-stressed bacteria from non-chlorinated secondary sewage effluents for use in the U.S. Environmental Protection Agency Alternate Test Procedure protocol

Janine R Hutchison; Mark W Widder; Shelby M Brooks; Linda M Brennan; Leah Souris; Valerie T Divito; William H van der Schalie; Richard M Ozanich

doi:10.1016/j.mimet.2019.105651

Consistent production of chlorine-stressed bacteria from non-chlorinated secondary sewage effluents for use in the U.S. Environmental Protection Agency Alternate Test Procedure protocol

J Microbiol Methods. 2019 Aug:163:105651. doi: 10.1016/j.mimet.2019.105651. Epub 2019 Jun 7.

Authors

Janine R Hutchison¹, Mark W Widder², Shelby M Brooks³, Linda M Brennan², Leah Souris², Valerie T Divito², William H van der Schalie², Richard M Ozanich³

Affiliations

¹ Chemical and Biological Signature Sciences Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA. Electronic address: janine.hutchison@pnnl.gov.
² US Army Center for Environmental Health Research, Fort Detrick, MD 21702-5000, USA.
³ Chemical and Biological Signature Sciences Group, National Security Directorate, Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA 99352, USA.

PMID: 31181230
DOI: 10.1016/j.mimet.2019.105651

Abstract

The U.S. Environmental Protection Agency Alternative Test Procedure protocol outlines a method to produce chlorine-stressed bacteria for water quality testing. Achieving consistent results is challenging due effluent variability. We describe a starting point for generating chlorine-stressed samples from secondary effluent to evaluate detection technologies to demonstrate comparability to EPA reference methods.

Keywords: Chlorination; Chlorine-stressed; EPA Alternate Test Procedure; Escherichia coli; Secondary sewage effluent; Total coliforms.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Chlorine / administration & dosage
Enterobacteriaceae / isolation & purification*
Halogenation
Sewage / microbiology*
United States
United States Environmental Protection Agency
Water Microbiology / standards
Water Purification / methods

Substances

Sewage
Chlorine