Phenolic compounds profile of different berry parts from novel Vitis vinifera L. red grape genotypes and Tempranillo using HPLC-DAD-ESI-MS/MS: A varietal differentiation tool

Abstract

The detailed phenolic composition of different berry parts from two novel V. vinifera L. red grape genotypes (Moribel and Tinto Fragoso), together the well-known Tempranillo, was established using high performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) over two consecutive vintages (2016 and 2017). More than 50 phenolic compounds were identified and quantified: 25 anthocyanins, 17 flavonols, 7 hydroxycinnamic acid derivatives, 2 stilbenes, and several flavan-3-ols. As far as we know, some anthocyanin and flavonol dihexosides were reported for the first time in V. vinifera L. grapes. Application of Principal Component Analysis (PCA) to experimental data showed a good separation of the novel grape genotypes and Tempranillo according to the phenolic profile of skins and seeds, mainly based on the proportion of trisubstituted anthocyanin derivatives, flavonols and flavan-3-ols, being a useful tool to differentiate these grape varieties.

Keywords: (+)-Catechin (PubChem CID: 9064); (−)-Epicatechin (PubChem CID: 72276); Cultivar differentiation; Grape berry parts; Grape genotype; HPLC-DAD-ESI-MS/MS; Malvidin 3-(6″-p-coumaroyl)-glucoside (PubChem CID: 131752272); Malvidin 3-glucoside (PubChem CID: 443652); Myricetin 3-glucoside (PubChem CID: 44259426); Phenolic composition; Procyanidin B1 (PubChem CID: 11250133); Quercetin 3-glucoside (PubChem CID: 5280804); Quercetin 3-glucuronide (PubChem CID: 11655911); Varietal authenticity; trans-Caftaric acid (PubChem CID: 6440397); trans-Piceid (PubChem CID: 5281718).