Direct effect of interleukin 2 on chronic lymphocytic leukaemia B cell functions and morphology

Clin Exp Immunol. 1987 Jun;68(3):677-84.

Abstract

The functional and morphological changes induced by recombinant interleukin 2 (IL-2) were studied in purified B cells from patients with untreated B chronic lymphocytic leukaemia (B-CLL). In eight of nine patients, purified B-CLL cells increased their DNA synthesis in response to IL-2 without preactivation in vitro. This response, studied in detail in three patients, was dose dependent and reached a maximum on day 5 or 6. IL-2 induced or increased IgM secretion in cultures from five of the nine patients studied. Two of this responsive group were particularly interesting as IL-2 not only stimulated IgM secretion but also induced the secretion of IgG. Immunoglobulin production was invariably monoclonal. B CLL cells incubated with IL-2 showed distinct morphological changes including an increase in the size of cytoplasm and enlargement of nuclei together with the appearance of nucleoli. These changes were present in all IL-2 treated cultures but were more pronounced in those containing immunoglobulin secreting cells. None of the IL-2 induced changes appeared to correlate with the clinical stage of the disease or the level of Tac antigen expression on the freshly isolated CLL B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism
  • B-Lymphocytes / pathology
  • Cells, Cultured
  • DNA, Neoplasm / biosynthesis
  • Dose-Response Relationship, Immunologic
  • Female
  • Humans
  • Immunoglobulin Light Chains / analysis
  • Immunoglobulins / biosynthesis
  • Interleukin-2 / immunology*
  • Leukemia, Lymphoid / blood
  • Leukemia, Lymphoid / immunology*
  • Male
  • Middle Aged

Substances

  • DNA, Neoplasm
  • Immunoglobulin Light Chains
  • Immunoglobulins
  • Interleukin-2