Interaction of water activity and temperature on the growth, gene expression and aflatoxin production by Aspergillus flavus on paddy and polished rice

Abstract

Water activity (a_w) and temperature are two pivotal environmental factors affecting Aspergillus flavus growth and aflatoxin production. Here, we found that AFB₁ production on polished rice can occur over a wider range of temperature × a_w levels than that on paddies. For fungal growth on polished rice, the optimum conditions were a_w 0.92-0.96 and 28-37 °C. The maximum amounts of AFB₁ on polished rice was observed at 33 °C and a_w 0.96. Compared to 33 °C, all tested genes of A. flavus on polished rice were significantly up-regulated at 25 °C under a_w 0.96. The late structural genes of pathway were significantly down-regulated at 37 °C under a_w 0.96, although aflR and aflS and most of early structural genes were up-regulated. Compared to a_w 0.96, most of pathway genes were significantly down-regulated at a_w 0.90 and 0.99 under 33 °C, although two regulatory genes were up-regulated at a_w 0.90.

Keywords: Aflatoxin; Aspergillus flavus; Rice; Temperature; Water activity (a(w)).