The Low-Dose ACTH Test: Usefulness of Combined Analysis of Serum and Salivary Maximum Cortisol Response in Pediatrics

J Clin Endocrinol Metab. 2019 Oct 1;104(10):4323-4330. doi: 10.1210/jc.2019-00304.

Abstract

Context: The low-dose (1 µg) ACTH test (LDT) is widely used to assess central adrenal insufficiency (CAI); however, the serum cortisol cutoff value is controversial. Salivary cortisol (SC) may be a more accurate measurement for CAI.

Objective: To assess a new maximum cutoff value of serum cortisol after LDT in pediatric patients, taking into account serum and SC measurements.

Design and setting: Prospective study in a pediatric tertiary referral center.

Working hypothesis: The combined analysis of serum and SC response to LDT might improve LDT for CAI diagnosis.

Participant and outcome measurement: A total of 145 pediatric patients underwent LDT. Serum and SC levels were measured. A central adrenal sufficient (CAS) response was established according to the reference serum cortisol cutoff value of ≥497 nmol/L.

Results: The LDT study showed central adrenal sufficiency in 72 patients and CAI in 73 patients. Considering the lower quartile of maximum SC value (21 nmol/L) in the CAS group, an intermediate CAI (InCAI) group and a real CAI (RCAI) group were defined. Regarding the median maximum value of serum cortisol levels in the InCAI group, a new serum cortisol cutoff value of 450 nmol/L was established. Furthermore, 91% of the patients in the RCAI group were below this cutoff value.

Conclusion: The combined evaluation of maximum serum and SC levels to LDT might be useful to define an InCAI group and to avoid unnecessary hormone replacement therapy. However, rigorous patient follow-up is required.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adrenal Insufficiency / blood
  • Adrenal Insufficiency / diagnosis*
  • Adrenocorticotropic Hormone / blood
  • Adrenocorticotropic Hormone / pharmacology*
  • Child
  • Cohort Studies
  • Female
  • Humans
  • Hydrocortisone / blood*
  • Male
  • Pediatrics
  • Pituitary-Adrenal System / drug effects
  • Pituitary-Adrenal System / physiopathology*
  • Prospective Studies
  • ROC Curve
  • Salivary Glands / metabolism*
  • Sensitivity and Specificity

Substances

  • Adrenocorticotropic Hormone
  • Hydrocortisone