A guide for drug inducible transcriptional activation with HIT systems

Chen Zhao; Shixian Wei; Yu Wang

doi:10.1016/bs.mie.2019.02.032

A guide for drug inducible transcriptional activation with HIT systems

Methods Enzymol. 2019:621:69-86. doi: 10.1016/bs.mie.2019.02.032. Epub 2019 Mar 15.

Authors

Chen Zhao¹, Shixian Wei², Yu Wang³

Affiliations

¹ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Electronic address: zhaochen@ioz.ac.cn.
² University of Chinese Academy of Sciences, Beijing, China.
³ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China. Electronic address: yuwang@post.harvard.edu.

PMID: 31128790
DOI: 10.1016/bs.mie.2019.02.032

Abstract

Precise investigation and manipulation of gene function often require modulation in a controlled and dynamic manner. In this chapter, we describe the methods to apply HIT systems for drug inducible transcriptional activation or simultaneous activation and genome editing in human cells. Together with those for editing, which are described in another chapter, HIT systems herein provide a valuable toolbox toward many biological applications, especially when precision and dynamics are required for a functional perturbation.

Keywords: CRISPR; Cas9; HIT-SunTag; HIT-TALE-SunTag; HIT2; TALE; Transcription activation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems
Cell Line
Gene Editing / methods*
Humans
Lentivirus / genetics
RNA, Guide, CRISPR-Cas Systems / genetics
Transcriptional Activation / drug effects*

Substances

RNA, Guide, CRISPR-Cas Systems