Synthesis, characterization, molecular docking and biological activities of novel pyrazoline derivatives

Arch Pharm (Weinheim). 2019 Jun;352(6):e1800359. doi: 10.1002/ardp.201800359. Epub 2019 May 24.

Abstract

In this study, synthesis of ethyl 2-((4-bromophenyl)diazenyl)-3-oxo-phenylpropanoate 1 was carried out and a series of new 3H-pyrazol-3-ones (P1-7) were synthesized from 1 as well as various hydrazines. The obtained yields of the synthesized compounds were moderate (40-70%) and these compounds were confirmed by spectral data. These novel pyrazoline derivatives were effective inhibitor compounds of the human carbonic anhydrase I and II isozymes (hCAs I and II) and of the acetylcholinesterase (AChE) enzyme, with Ki values in the range of 17.4-40.7 nM for hCA I, 16.1-55.2 nM for hCA II, and 48.2-84.1 nM for AChE. In silico studies were performed on the compounds inhibiting hCA I, hCA II, and AChE receptors. On the basis of the findings, the inhibition profile of the new pyrazoline compounds at the receptors was determined.

Keywords: acetylcholinesterase; enzyme inhibition; human carbonic anhydrase; in silico study; induced fit docking; pyrazoline.

MeSH terms

  • Acetylcholinesterase / metabolism
  • Binding Sites
  • Carbonic Anhydrase I / antagonists & inhibitors
  • Carbonic Anhydrase II / antagonists & inhibitors
  • Carbonic Anhydrase Inhibitors / chemical synthesis*
  • Carbonic Anhydrase Inhibitors / chemistry
  • Carbonic Anhydrase Inhibitors / pharmacology
  • Cholinesterase Inhibitors / chemical synthesis*
  • Cholinesterase Inhibitors / chemistry
  • Cholinesterase Inhibitors / pharmacology
  • Computer Simulation
  • Drug Design*
  • Humans
  • Molecular Docking Simulation
  • Molecular Structure
  • Pyrazoles / chemical synthesis*
  • Pyrazoles / chemistry
  • Pyrazoles / pharmacology
  • Structure-Activity Relationship

Substances

  • Carbonic Anhydrase Inhibitors
  • Cholinesterase Inhibitors
  • Pyrazoles
  • Acetylcholinesterase
  • Carbonic Anhydrase I
  • Carbonic Anhydrase II