Study of 109 Achromobacter spp. isolates from 9 French CF centres reveals the circulation of a multiresistant clone of A. xylosoxidans belonging to ST 137

Lucie Amoureux; Juliette Sauge; Benoit Sarret; Matthieu Lhoumeau; Audrey Bajard; Jennifer Tetu; Julien Bador; Catherine Neuwirth; MucoMicrobes group

doi:10.1016/j.jcf.2019.04.005

Study of 109 Achromobacter spp. isolates from 9 French CF centres reveals the circulation of a multiresistant clone of A. xylosoxidans belonging to ST 137

J Cyst Fibros. 2019 Nov;18(6):804-807. doi: 10.1016/j.jcf.2019.04.005. Epub 2019 May 16.

Authors

Lucie Amoureux¹, Juliette Sauge², Benoit Sarret², Matthieu Lhoumeau², Audrey Bajard², Jennifer Tetu², Julien Bador³, Catherine Neuwirth³; MucoMicrobes group

Collaborators

MucoMicrobes group:
Jocelyne Caillon, Emilie Cardot-Martin, Vincent Cattoir, Anne Doléans-Jordheim, Agnès Ferroni, Hélène Guet-Revillet, Geneviève Héry-Arnaud, Christine Segonds, Elise Thomas, Patrick Plésiat, Hoang Vu-Thien

Affiliations

¹ Department of Bacteriology, University Hospital of Dijon, BP 37013, 21070, Dijon Cedex, France; UMR/CNRS 6249 Chrono-environnement, University of Franche-Comté, Besançon, France. Electronic address: lucie.amoureux@chu-dijon.fr.
² Department of Bacteriology, University Hospital of Dijon, BP 37013, 21070, Dijon Cedex, France.
³ Department of Bacteriology, University Hospital of Dijon, BP 37013, 21070, Dijon Cedex, France; UMR/CNRS 6249 Chrono-environnement, University of Franche-Comté, Besançon, France.

PMID: 31104975
DOI: 10.1016/j.jcf.2019.04.005

Abstract

We previously reported the distribution of Achromobacter spp. (species and Sequence Types (ST)) in our French Cystic Fibrosis (CF) centre. In the present study we collected 109 Achromobacter isolates (1/patient) from 9 other French CF Centres for species identification, antimicrobial susceptibility testings and Multilocus-Sequence-Typing (MLST) analysis. Ten species were detected, A. xylosoxidans being the most predominant one (73.4% of the isolates). Piperacillin-tazobactam, ceftazidime, imipenem, meropenem and ciprofloxacin were respectively active against 88, 70, 79, 72 and 23% of the isolates. Among the 79 A. xylosoxidans isolates, 46 STs were detected. Interestingly, ST 137, recovered in 4 centres (5 patients), was previously detected in our centre (2 patients). The strains from the 7 patients belonged to the same pulsotype (pulsed-field-gel-electrophoresis analysis) and harboured acquired resistance to meropenem, ceftazidime, ciprofloxacin, and except for 2 isolates, to imipenem and piperacillin-tazobactam. This is the first description in France of a circulating multiresistant A. xylosoxidans strain.

Keywords: Achromobacter; Cystic fibrosis; Identification; MLST; Resistance; ST 137; nrdA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Achromobacter denitrificans* / drug effects
Achromobacter denitrificans* / isolation & purification
Anti-Bacterial Agents* / classification
Anti-Bacterial Agents* / pharmacology
Cystic Fibrosis* / drug therapy
Cystic Fibrosis* / epidemiology
Cystic Fibrosis* / microbiology
DNA, Bacterial / isolation & purification
Drug Resistance, Multiple, Bacterial
Electrophoresis, Gel, Pulsed-Field / methods
France / epidemiology
Gram-Negative Bacterial Infections* / epidemiology
Gram-Negative Bacterial Infections* / microbiology
Humans
Microbial Sensitivity Tests / methods
Microbial Sensitivity Tests / statistics & numerical data
Multilocus Sequence Typing / methods

Substances

Anti-Bacterial Agents
DNA, Bacterial