Abstract
Metabolic engineers endeavor to create a bio-based manufacturing industry using microbes to produce fuels, chemicals, and medicines. Plant natural products (PNPs) are historically challenging to produce and are ubiquitous in medicines, flavors, and fragrances. Engineering PNP pathways into new hosts requires finding or modifying a suitable host to accommodate the pathway, planning and implementing a biosynthetic route to the compound, and discovering or engineering enzymes for missing steps. In this review, we describe recent developments in metabolic engineering at the level of host, pathway, and enzyme, and discuss how the field is approaching ever more complex biosynthetic opportunities.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Biological Products / metabolism*
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Biosynthetic Pathways / genetics
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Enzymes / genetics
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Enzymes / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Metabolic Engineering / methods*
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Metabolic Engineering / trends
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Microorganisms, Genetically-Modified / genetics
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Microorganisms, Genetically-Modified / metabolism*
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Plants / metabolism*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Synthetic Biology / methods
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Synthetic Biology / trends
Substances
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Biological Products
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Enzymes
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Escherichia coli Proteins
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Saccharomyces cerevisiae Proteins