[Evaluation of the results of a direct solid-phase immunoenzyme method for determining antigens]

Vopr Virusol. 1986 Sep-Oct;31(5):573-7.
[Article in Russian]

Abstract

Aspects of an objective analysis of the results of solid-phase enzyme-immunoassay (SPEIA) for identification of antigens (a direct version) were considered on the model of the virus of hemorrhagic fever with renal syndrome. Using a statistical method, certain parameters of 11 types of polystyrene and polyvinylchloride microplates (solid carrier) of different manufacturers were studied from the point of view of their suitability for obtaining reproducible SPEIA results. Statistical methods for the evaluation of EIA data are proposed to increase the effectiveness of the use of this test and making it quite an objective one.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Animals
  • Antigens, Viral / analysis*
  • Evaluation Studies as Topic
  • Immunoenzyme Techniques* / instrumentation
  • Lung / immunology
  • Orthohantavirus / immunology*
  • Quality Control
  • Rodentia
  • Statistics as Topic

Substances

  • Antigens, Viral