Successful Whole Genome Sequencing-guided Treatment of Mycoplasma hominis Ventriculitis in a Preterm Infant

Pediatr Infect Dis J. 2019 Jul;38(7):749-751. doi: 10.1097/INF.0000000000002306.

Abstract

We report a case of Mycoplasma hominis ventriculitis in a preterm neonate that was successfully identified with 16S ribosomal RNA sequencing and whole genome sequencing after failure to detect the pathogen with conventional diagnostic methods. The infant required doxycycline with subsequent clearance of the infection and no evidence of drug toxicity.

Publication types

  • Case Reports
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anti-Bacterial Agents / administration & dosage*
  • Cerebral Ventriculitis / diagnosis*
  • Cerebral Ventriculitis / drug therapy*
  • Cerebral Ventriculitis / microbiology
  • Cerebral Ventriculitis / pathology
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Doxycycline / administration & dosage*
  • Female
  • Humans
  • Infant, Newborn
  • Infant, Premature
  • Male
  • Mycoplasma Infections / diagnosis*
  • Mycoplasma Infections / drug therapy*
  • Mycoplasma Infections / microbiology
  • Mycoplasma Infections / pathology
  • Mycoplasma hominis / classification
  • Mycoplasma hominis / genetics
  • Mycoplasma hominis / isolation & purification*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Treatment Outcome
  • Whole Genome Sequencing

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Doxycycline