Objective: CRP gene polymorphism is common in inflammatory diseases, but such association has not been reported in periodontitis. Our objective was to interrogate SNPs of crp in chronic periodontitis in a case-control manner.
Methods: DNAs were extracted from mouthwash samples of 116 volunteers using salting-out method. Selected 12 5'UTR SNPs of crp were genotyped using ARMS-PCR.
Results: TC genotype of - 757T > C polymorphism (rs3093059) showed protective association (OR- 0.29, 95%CI-0.12-0.68, and p-0.004), and wild type - 757TT showed susceptible association with a p value of 0.008 (OR-3.09, 95%CI-1.33-7.15).
Conclusion: The observation of protective and susceptible association of crp - 757T > C polymorphism may be useful for better management and prophylaxis of periodontitis.
Keywords: Amplification refractory mutation system (ARMS)–PCR; C-reactive protein (CRP); Chronic periodontitis (CP); Single-nucleotide polymorphism (SNP).