DNA Extraction from Keratin and Chitin

Paula F Campos; M Thomas P Gilbert

doi:10.1007/978-1-4939-9176-1_7

DNA Extraction from Keratin and Chitin

Methods Mol Biol. 2019:1963:57-63. doi: 10.1007/978-1-4939-9176-1_7.

Authors

Paula F Campos¹, M Thomas P Gilbert²

Affiliations

¹ Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark.
² Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark. mtpgilbert@gmail.com.

PMID: 30875044
DOI: 10.1007/978-1-4939-9176-1_7

Abstract

DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica column or organic methods.

Keywords: Ancient DNA; Chitinous tissue; Cuticle; DNA extraction; Exoskeleton; Feathers; Hair; Keratinous tissue; Nail; Silica.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chitin / chemistry*
DNA / analysis*
DNA / isolation & purification*
Endopeptidase K / metabolism
Humans
Keratins / chemistry*
Silicon Dioxide / chemistry

Substances

Chitin
Keratins
Silicon Dioxide
DNA
Endopeptidase K