Molecular cloning and characterization of the pig MHC class Ⅰ-related MR1 gene

Xingxing Xiao; Baohong Liu; Xueting Ma; Shunli Yang; Jianping Cai

doi:10.1016/j.dci.2019.02.020

Molecular cloning and characterization of the pig MHC class Ⅰ-related MR1 gene

Dev Comp Immunol. 2019 Jul:96:58-67. doi: 10.1016/j.dci.2019.02.020. Epub 2019 Mar 2.

Authors

Xingxing Xiao¹, Baohong Liu¹, Xueting Ma¹, Shunli Yang¹, Jianping Cai²

Affiliations

¹ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province, 730046, People's Republic of China; Jiangsu Co-Innovation Center for Prevention and Control of Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province, 225009, People's Republic of China.
² State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province, 730046, People's Republic of China; Jiangsu Co-Innovation Center for Prevention and Control of Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu Province, 225009, People's Republic of China. Electronic address: caijianping@caas.cn.

PMID: 30836125
DOI: 10.1016/j.dci.2019.02.020

Abstract

Major histocompatibility complex (MHC) class Ⅰ-related protein 1 (MR1), the most highly conserved MHC class Ⅰ molecule among mammals, is the restricting molecule for mucosal-associated invariant T (MAIT) cells. MAIT cells, a novel subset of T cells, play important roles in modulating the immune responses to infectious and non-infectious diseases, and recognize antigens in the context of MR1. MR1 has been identified in many species, including human, mouse, sheep, and cow. Here, we cloned and characterized pig (Sus scrofa) MR1 (pMR1) transcripts, including five unique splice variants, from pig peripheral blood mononuclear cell cDNA. We also examined the tissue distribution of pMR1 and confirmed reactivity of pMR1 using a MR1 specific monoclonal antibody 26.5, demonstrating that the pMR1 gene was expressed in all tested tissues. Finally, we predicted the pMR1 3D structure and analyzed the docking mode of the MR1-5-OP-RU complex, finding that the docking mode of pMR1 with 5-OP-RU is similar to human MR1 docking. Collectively, this description of pMR1 adds to our understanding of the evolution of MHC molecules, and provides a theoretical basis for the subsequent study of pig MAIT cells.

Keywords: I -related protein 1 (MR1); MAIT cells; MHC class; Pig.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cloning, Molecular
Genes, MHC Class I / genetics*
Genes, MHC Class I / immunology
Histocompatibility Antigens Class I / genetics*
Histocompatibility Antigens Class I / immunology
Ligands
Male
Minor Histocompatibility Antigens / genetics*
Minor Histocompatibility Antigens / immunology
Molecular Docking Simulation
Primary Cell Culture
Sequence Alignment
Sequence Homology, Amino Acid
Sus scrofa / genetics
Sus scrofa / immunology*

Substances

Histocompatibility Antigens Class I
Ligands
MR1 protein, human
Minor Histocompatibility Antigens