Target-assisted self-cleavage DNAzyme probes for multicolor simultaneous imaging of tumor-related microRNAs with signal amplification

Chem Commun (Camb). 2019 Mar 18;55(22):3278-3281. doi: 10.1039/c9cc00451c. Epub 2019 Feb 27.

Abstract

In this work, we have developed a novel and highly selective signal amplification strategy based on target-assisted self-cleavage DNAzyme probes for multicolor and simultaneous imaging of miRNA-222 and miRNA-223 in living cells. The fluctuation of miRNA-222 and miRNA-223 expression levels could also be monitored.

MeSH terms

  • Cell Line, Tumor
  • Color
  • DNA, Catalytic / chemistry*
  • Humans
  • Hydrolysis
  • MicroRNAs / chemistry*
  • Molecular Probes / chemistry*
  • Nucleic Acid Amplification Techniques / methods*
  • RNA, Neoplasm / genetics*

Substances

  • DNA, Catalytic
  • MIRN222 microRNA, human
  • MIRN223 microRNA, human
  • MicroRNAs
  • Molecular Probes
  • RNA, Neoplasm