Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A

Rev Soc Bras Med Trop. 2019 Feb 14:52:e20180001. doi: 10.1590/0037-8682-0001-2018.

Abstract

Introduction: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival.

Methods: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host.

Results: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture.

Conclusions: These findings highlight the role of host stress in the virulence of S. epidermidis.

MeSH terms

  • Biofilms / growth & development*
  • Biological Assay
  • Culture Media / pharmacology*
  • Host Microbial Interactions / drug effects
  • Host Microbial Interactions / physiology*
  • Humans
  • Staphylococcus epidermidis / drug effects
  • Staphylococcus epidermidis / pathogenicity
  • Staphylococcus epidermidis / physiology*
  • Stress, Physiological
  • Virulence / drug effects
  • Virulence / physiology*

Substances

  • Culture Media