Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment

Jianliang Xue; Yanan Wu; Ke Shi; Xinfeng Xiao; Yu Gao; Lin Li; Yanlu Qiao

doi:10.1016/j.biortech.2019.02.019

Study on the degradation performance and kinetics of immobilized cells in straw-alginate beads in marine environment

Bioresour Technol. 2019 May:280:88-94. doi: 10.1016/j.biortech.2019.02.019. Epub 2019 Feb 4.

Authors

Jianliang Xue¹, Yanan Wu², Ke Shi², Xinfeng Xiao², Yu Gao², Lin Li², Yanlu Qiao²

Affiliations

¹ College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong 266590, China. Electronic address: ll-1382@163.com.
² College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong 266590, China.

PMID: 30763865
DOI: 10.1016/j.biortech.2019.02.019

Abstract

In this study, two strains Halomonas and Aneurinibacillus were mixed in equal proportions as free cells that could degrade diesel and produce biosurfactant. A new type of immobilized cells, free cells immobilized in beads combined with sodium alginate and straw, was studied. The components of straw-alginate beads were optimized by Response Surface Method, and the degradation performance of immobilized cells was determined. The result indicated that the density, strength and broken rate of straw-alginate beads were 1.04 g/cm³, 216 g and 4%, respectively. The best degradation rate of immobilized cells in straw-alginate beads could be 68.68%. Lately, by analyzing the Monod model, v_max (maximum specific degradation rate of diesel) and K_S (half saturation rate constant) of immobilized cells in straw-alginate beads were 1.84 d^-1 and 3.23 g/L, respectively, which explained the higher degradation performance.

Keywords: Biodegradation; Degradation kinetics; Degradation rate; Immobilized cells in straw-alginate beads.

MeSH terms

Alginates / metabolism*
Cells, Immobilized
Kinetics

Substances

Alginates