Pedobacter pollutisoli sp. nov., Isolated from Tetrabromobisphenol A-Contaminated Soil

Abstract

A Gram-stain negative, strictly aerobic, non-spore forming, non-motile, rod-shaped bacterium, designated TBBPA-24^T, was isolated from tetrabromobisphenol A-contaminated soil in China. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain TBBPA-24^T was most closely related to Pedobacter nanyangensis Q4^T (96.5%) and Pedobacter 'zeaxanthinifaciens' TDMA-5^T (96.1%). Chemotaxonomic analysis revealed that strain TBBPA-24^T possessed MK-7 as the major respiratory quinone and lipid, aminolipid, phospholipid, phosphatidylethanolamine, and phosphoaminolipid as the major polar lipid. The major fatty acids were iso-C_15:0 (40.2%), summed feature 3 (C_16:1ω6c and/or C_16:1ω7c, 25.6%) and iso-C_17:0 3-OH (16.4%). The genomic DNA G+C content of strain TBBPA-24^T was 43.9 mol%. Based on the phylogenetic, phenotypic characteristics, and chemotaxonomic data, strain TBBPA-24^T is considered a novel species of the genus Pedobacter, for which the name Pedobacter pollutisoli sp. nov. is proposed. The type strain TBBPA-24^T (= KCTC 62314^T = CCTCC AB 2017244^T) is proposed.