Toolbox for In Vivo Imaging of Host-Parasite Interactions at Multiple Scales

Mariana De Niz; Florentin Spadin; Matthias Marti; Jens V Stein; Martin Frenz; Friedrich Frischknecht

doi:10.1016/j.pt.2019.01.002

Toolbox for In Vivo Imaging of Host-Parasite Interactions at Multiple Scales

Trends Parasitol. 2019 Mar;35(3):193-212. doi: 10.1016/j.pt.2019.01.002. Epub 2019 Feb 10.

Authors

Mariana De Niz¹, Florentin Spadin², Matthias Marti³, Jens V Stein⁴, Martin Frenz⁵, Friedrich Frischknecht⁶

Affiliations

¹ Institute of Cell Biology - Heussler Laboratory, University of Bern, Bern, Switzerland; Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow, Scotland, UK; Current affiliation: Instituto de Medicina Molecular - João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal; These authors contributed equally. Electronic address: mariana.deniz@medicina.ulisboa.pt.
² Institute of Applied Physics, University of Bern, Bern, Switzerland; These authors contributed equally.
³ Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow, Scotland, UK.
⁴ Theodor Kocher Institute, University of Bern, Bern, Switzerland; Department of Oncology, Microbiology and Immunology, University of Fribourg, Fribourg, Switzerland.
⁵ Institute of Applied Physics, University of Bern, Bern, Switzerland.
⁶ Integrative Parasitology, Centre for Infectious Diseases, Heidelberg University Medical School, Heidelberg, Germany.

PMID: 30745251
DOI: 10.1016/j.pt.2019.01.002

Abstract

Animal models have for long been pivotal for parasitology research. Over the last few years, techniques such as intravital, optoacoustic and magnetic resonance imaging, optical projection tomography, and selective plane illumination microscopy developed promising potential for gaining insights into host-pathogen interactions by allowing different visualization forms in vivo and ex vivo. Advances including increased resolution, penetration depth, and acquisition speed, together with more complex image analysis methods, facilitate tackling biological problems previously impossible to study and/or quantify. Here we discuss advances and challenges in the in vivo imaging toolbox, which hold promising potential for the field of parasitology.

Keywords: animal models; imaging; in vivo; parasitology.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Host-Parasite Interactions*
Intravital Microscopy* / methods
Intravital Microscopy* / trends
Parasitology* / methods
Parasitology* / standards
Parasitology* / trends
Tomography, Optical