The Possible Role of Complete Loss of Myostatin in Limiting Excessive Proliferation of Muscle Cells (C2C12) via Activation of MicroRNAs

Peixuan Huang; Daxin Pang; Kankan Wang; Aishi Xu; Chaogang Yao; Mengjing Li; Wenni You; Qiushuang Wang; Hao Yu

doi:10.3390/ijms20030643

The Possible Role of Complete Loss of Myostatin in Limiting Excessive Proliferation of Muscle Cells (C2C12) via Activation of MicroRNAs

Int J Mol Sci. 2019 Feb 2;20(3):643. doi: 10.3390/ijms20030643.

Authors

Peixuan Huang¹, Daxin Pang², Kankan Wang³, Aishi Xu⁴, Chaogang Yao⁵, Mengjing Li⁶, Wenni You⁷, Qiushuang Wang⁸, Hao Yu⁹

Affiliations

¹ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. huangpx16@mails.jlu.edu.cn.
² Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. pdx@jlu.edu.cn.
³ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. wang4196@purdue.edu.
⁴ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. xuas14@mails.jlu.edu.cn.
⁵ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. yaocg13@mails.jlu.edu.cn.
⁶ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. mjli18@mails.jlu.edu.cn.
⁷ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. youwn18@mails.jlu.edu.cn.
⁸ Public Computer Education and Research Center, Jilin University, Changchun 130062, China. wqs@jlu.edu.cn.
⁹ Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. yu_hao@jlu.edu.cn.

Abstract

Myostatin (MSTN) is a member of the TGF-β superfamily that negatively regulates skeletal muscle growth and differentiation. However, the mechanism by which complete MSTN deletion limits excessive proliferation of muscle cells remains unclear. In this study, we knocked out MSTN in mouse myoblast lines using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) system and sequenced the mRNA and miRNA transcriptomes. The results show that complete loss of MSTN upregulates seven miRNAs targeting an interaction network composed of 28 downregulated genes, including TGFB1, FOS and RB1. These genes are closely associated with tumorigenesis and cell proliferation. Our study suggests that complete loss of MSTN may limit excessive cell proliferation via activation of miRNAs. These data will contribute to the treatment of rhabdomyosarcoma (RMS).

Keywords: C2C12; MSTN; RMS; RNA-seq; miRNA-seq; transcriptomics.

MeSH terms

Animals
Cell Line
Computational Biology / methods
Gene Expression Profiling
Gene Expression Regulation
Gene Knockout Techniques
Gene Order
Gene Regulatory Networks
Genetic Vectors / genetics
Mice
MicroRNAs / genetics*
Molecular Sequence Annotation
Muscle Cells / metabolism*
Myostatin / deficiency*
RNA Interference
RNA, Messenger / genetics
Reproducibility of Results

Substances

MicroRNAs
Myostatin
RNA, Messenger

Abstract

MeSH terms

Substances

Grants and funding