Spontaneously formed porous structure and M1 polarization effect of Fe3O4 nanoparticles for enhanced antitumor therapy

Abstract

In this study, we developed a novel Fe₃O₄ nanoparticles-doxorubicin (DOX)-Hyaluronic acid (HA) nanoparticles on the basis of firstly discovered "formed porous structure" in spontaneously assembled Fe₃O₄ nanoparticles. The Mechanism of Action (MOA) behind this porous DOX-loading cargo was tested and confirmed. A multi-functional Fe₃O₄-DOX+HA nanoparticle was further constructed by incorporating HA into our system. In vitro and in vivo studies exhibited that Fe₃O₄-DOX+HA owned enhanced antitumor efficacy with significantly prolonged survival time due to the combination of M₁ polarization ability of Fe₃O₄ nanoparticles, tumor killing effect of DOX and tumor and TAM-targeting effect of HA. All in all, our studies offer a novel strategy to develop a multifunctional antitumor system with a simple preparation method and an enhanced therapeutic outcome.

Keywords: M(1) polarization; Porous structure; Spontaneous assembled Fe(3)O(4) nanoparticles; Tumor and TAM targeting.