Single-Molecule Analysis with Solid-State Nanopores

Annu Rev Anal Chem (Palo Alto Calif). 2019 Jun 12;12(1):371-387. doi: 10.1146/annurev-anchem-061417-125903. Epub 2019 Feb 1.

Abstract

Solid-state nanopores and nanopipettes are an exciting class of single-molecule sensors that has grown enormously over the last two decades. They offer a platform for testing fundamental concepts of stochasticity and transport at the nanoscale, for studying single-molecule biophysics and, increasingly, also for new analytical applications and in biomedical sensing. This review covers some fundamental aspects underpinning sensor operation and transport and, at the same time, it aims to put these into context as an analytical technique. It highlights new and recent developments and discusses some of the challenges lying ahead.

Keywords: Coulter; DNA; nanopipette; nanopore; resistive pulse sensing; sensing.