Semisynthesis of an Anticancer DPAGT1 Inhibitor from a Muraymycin Biosynthetic Intermediate

Org Lett. 2019 Feb 15;21(4):876-879. doi: 10.1021/acs.orglett.8b03716. Epub 2019 Jan 30.

Abstract

We have explored a method to convert a muraymycin biosynthetic intermediate 3 to an anticancer drug lead 2 for in vivo and thorough preclinical studies. Cu(OAc)2 forms a stable complex with the amide 4 and prevents electrophilic reactions at the 2-((3-aminopropyl)amino)acetamide moiety. Under the present conditions, the desired 5″-primary amine was selectively protected with (Boc)2O to yield 6. The intermediate 6 was converted to 2 in two steps with 90% yield.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemical synthesis*
  • Antineoplastic Agents / pharmacology
  • Benzamides / chemistry*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / metabolism
  • Enzyme Inhibitors / pharmacology*
  • Humans
  • Molecular Conformation
  • N-Acetylglucosaminyltransferases / antagonists & inhibitors*
  • N-Acetylglucosaminyltransferases / metabolism
  • Nucleosides / chemistry*
  • Nucleosides / metabolism
  • Nucleotides / chemistry
  • Peptides / chemistry
  • Phenylurea Compounds / chemical synthesis*
  • Phenylurea Compounds / pharmacology
  • Piperidines / chemical synthesis*
  • Piperidines / pharmacology
  • Structure-Activity Relationship
  • Urea / chemistry
  • Uridine / analogs & derivatives*
  • Uridine / chemical synthesis
  • Uridine / pharmacology

Substances

  • 1-(4-amidinophenyl)-3-(4-phenoxyphenyl)urea
  • Antineoplastic Agents
  • Benzamides
  • Enzyme Inhibitors
  • Nucleosides
  • Nucleotides
  • Peptides
  • Phenylurea Compounds
  • Piperidines
  • aminouridyl phenoxypiperidinbenzyl urea
  • muraymycin A1
  • Urea
  • N-Acetylglucosaminyltransferases
  • dolichyl-phosphate alpha-N-acetylglucosaminyltransferase
  • Uridine