A novel NIR-emissive probe with large Stokes shift for hypochlorite detection and imaging in living cells

Jingwei Liu; Zheng Yin

doi:10.1016/j.talanta.2018.12.086

A novel NIR-emissive probe with large Stokes shift for hypochlorite detection and imaging in living cells

Talanta. 2019 May 1:196:352-356. doi: 10.1016/j.talanta.2018.12.086. Epub 2018 Dec 26.

Authors

Jingwei Liu¹, Zheng Yin²

Affiliations

¹ Center of Basic Molecular Science, Department of chemistry, Tsinghua University, Beijing 100084, China.
² Center of Basic Molecular Science, Department of chemistry, Tsinghua University, Beijing 100084, China. Electronic address: yinzheng@mail.tsinghua.edu.cn.

PMID: 30683375
DOI: 10.1016/j.talanta.2018.12.086

Abstract

Due to the importance of hypochlorite (ClO^-) in the process of life, a near-infrared region (NIR) emissive probe (DCPO-DMTC) with large Stokes shift was synthesized for the selective detection of hypochlorite. This probe detects exogenous and endogenous hypochlorite via "oxidative deprotection" of dimethylthiocarbamate-protected phenolic hydroxyl groups. The response was monitored by time-course UV-Vis and fluorescent spectroscopy. The emission response of the probe to ClO^- presented a good linear relationship in the 0-100 µM concentration range, and the LOD of this probe was 164 nM. The probe was used to successfully visualize endogenous ClO^- generation in RAW 246.7 cells under external stimulation.

Keywords: Bioimaging; Hypochlorote; Large Stokes-shift; NIR-emissive.

MeSH terms

Animals
Fluorescent Dyes / chemistry*
Humans
Hypochlorous Acid / analysis*
Hypochlorous Acid / chemistry
Mice
RAW 264.7 Cells
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Thiocarbamates / chemistry

Substances

Fluorescent Dyes
Thiocarbamates
dimethylthiocarbamate
Hypochlorous Acid