A water-soluble benzoxazole-based probe: Real-time monitoring PPi via situ reaction by two-photon cells imaging

Dan Liu; Sheng Liu; Xinyu Zhang; Qiong Zhang; Jianhua Yu; Mingdi Yang; Xingyuan Yang; Yupeng Tian; Xiaojiao Zhu; Hongping Zhou

doi:10.1016/j.talanta.2018.11.036

A water-soluble benzoxazole-based probe: Real-time monitoring PPi via situ reaction by two-photon cells imaging

Talanta. 2019 Apr 1:195:158-164. doi: 10.1016/j.talanta.2018.11.036. Epub 2018 Nov 14.

Authors

Affiliations

¹ College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, 230601 Hefei, PR China.
² College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, 230601 Hefei, PR China; School of Materials and Chemical Engineering, Anhui Jianzhu University, Hefei 230601, PR China.
³ Faculty of Health Sciences, Anhui University, Hefei 230601, PR China.
⁴ College of Chemistry and Chemical Engineering, Anhui University and Key Labotatory of Functional Inorganic Materials Chemistry of Anhui Province, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, 230601 Hefei, PR China. Electronic address: zhpzhp@263.net.

PMID: 30625526
DOI: 10.1016/j.talanta.2018.11.036

Abstract

Pyrophosphate (PPi) played crucial roles in various fundamental physiological processes. Herein, a two-photon absorption (TPA) "On-Off-On" type benzoxazole-based fluorescence probe BN was designed and synthesized, which detected PPi through Cu²⁺ displacing method in situ system in aqueous medium. The on-off-on process of BN recognizing PPi was verified by mass spectra and theoretical calculations, which was successfully applied in TPA cells imaging.

Keywords: Benzoxazole; Fluorescence probe; LLCT and MLCT; TPA imaging.

MeSH terms

Benzoxazoles / chemistry*
Coordination Complexes / chemistry*
Copper / chemistry*
Diphosphates / analysis*
Diphosphates / chemistry
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Optical Imaging
Solubility
Water / chemistry

Substances

Benzoxazoles
Coordination Complexes
Diphosphates
Fluorescent Dyes
Water
Copper