Matrilin-2 prevents the TNFα-induced apoptosis of WB-F344 cells via suppressing JNK pathway

Biotechnol Appl Biochem. 2019 May;66(3):309-315. doi: 10.1002/bab.1726. Epub 2019 Jan 29.

Abstract

Oval cells, a kind of hepatic progenitor cell quiescent at normal condition, activates to proliferate and differentiate into hepatocytes under severe and long-term liver injury, which usually raises severe inflammation. However, how oval cell survives in the inflammatory milieu interne is still unclear. Tumor necrosis factor α (TNFα), mimicking inflammatory hepatic milieu interne, was used to treat oval cell line, WB-F344, to test the protective function of matrilin-2. In this study, our data suggested that matrilin-2 prevented TNFα-induced apoptosis in WB-F344 cells via inhibiting ASK1/MKK7/JNK pathway. In conclusion, we determined that matrilin-2 plays the key role in maintaining the survival of oval cell and guarantees its proliferation under various injury factors.

Keywords: JNK pathway; matrilin-2; oval cell; proliferation.

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Cell Survival / drug effects
  • Cells, Cultured
  • JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors*
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Matrilin Proteins / metabolism
  • Rats
  • Rats, Inbred F344
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Matn2 protein, rat
  • Matrilin Proteins
  • Tumor Necrosis Factor-alpha
  • JNK Mitogen-Activated Protein Kinases