Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus

Stefanie Schuster; Srinethe Saravanakumar; Ludger Schöls; Stefan Hauser

doi:10.1016/j.scr.2018.101378

Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus

Stem Cell Res. 2019 Jan:34:101378. doi: 10.1016/j.scr.2018.101378. Epub 2018 Dec 21.

Authors

Stefanie Schuster¹, Srinethe Saravanakumar², Ludger Schöls³, Stefan Hauser⁴

Affiliations

¹ Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Graduate School of Cellular and Molecular Neuroscience, University of Tübingen, Tübingen, Germany.
² Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany.
³ Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany.
⁴ German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany. Electronic address: stefan.hauser@dzne.de.

PMID: 30605842
DOI: 10.1016/j.scr.2018.101378

Abstract

STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency.

MeSH terms

Adult
CRISPR-Cas Systems / genetics*
Cell Culture Techniques / methods*
Cell Line
Female
Gene Knockout Techniques*
Genetic Loci*
Homozygote
Humans
Induced Pluripotent Stem Cells / cytology*
Ubiquitin-Protein Ligases / genetics*

Substances

STUB1 protein, human
Ubiquitin-Protein Ligases