A novel SERS-based aptasensor for ultrasensitive sensing of microcystin-LR

Deyun He; Zhengzong Wu; Bo Cui; Zhengyu Jin

doi:10.1016/j.foodchem.2018.11.071

A novel SERS-based aptasensor for ultrasensitive sensing of microcystin-LR

Food Chem. 2019 Apr 25:278:197-202. doi: 10.1016/j.foodchem.2018.11.071. Epub 2018 Nov 13.

Authors

Deyun He¹, Zhengzong Wu², Bo Cui³, Zhengyu Jin⁴

Affiliations

¹ State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
² State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China. Electronic address: wzzqlu@hotmail.com.
³ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China. Electronic address: cuib@qlu.edu.cn.
⁴ State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

PMID: 30583362
DOI: 10.1016/j.foodchem.2018.11.071

Abstract

We developed a novel aptasensor based on surface enhanced Raman spectroscopy (SERS) and applied it for highly sensitive detection of microcystin-LR (MC-LR). In this work, MC-LR aptamer and its corresponding complementary DNA fragments (cDNA) were conjugated to gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), respectively. Then, MC-LR aptamer-AuNPs and cDNA-MNPs conjugates were used as signal probes and capture probes, respectively. The proposed assay exhibited a linearity range from 0.01 to 200 ng/mL with the limit of detection (LOD) of 0.002 ng/mL. In addition, the reliability of the novel approach was validated by detecting different levels of MC-LR spiked in tap water samples. Overall, the novel aptasensor paves a new way for rapid and accurate determination of MC-LR and can be referred to detect other hazardous substances in water products.

Keywords: Food analysis; Magnetic nanoparticles; Microcystin-LR; Rapid detection; Surface enhanced Raman spectroscopy.

MeSH terms

Aptamers, Nucleotide / metabolism*
Biosensing Techniques / methods*
Color
DNA, Complementary / chemistry
Gold / chemistry
Limit of Detection*
Marine Toxins
Metal Nanoparticles / chemistry
Microcystins / analysis*
Microcystins / metabolism
Reproducibility of Results
Spectrum Analysis, Raman*
Water / chemistry

Substances

Aptamers, Nucleotide
DNA, Complementary
Marine Toxins
Microcystins
Water
Gold
cyanoginosin LR