Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization

Huanhuan Joyce Chen; Michael L Shuler

doi:10.1007/978-1-4939-8967-6_7

Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization

Methods Mol Biol. 2019:1907:91-102. doi: 10.1007/978-1-4939-8967-6_7.

Authors

Huanhuan Joyce Chen^{1

2}, Michael L Shuler³

Affiliations

¹ Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
² Meyer Cancer Center, Weill Cornell Medicine, Cornell University, New York, NY, USA.
³ Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA. mls50@cornell.edu.

PMID: 30542993
DOI: 10.1007/978-1-4939-8967-6_7

Abstract

The tissue engineering method of decellularization and recellularization has been successfully used in a variety of regenerative medicine applications. The protocols used to de/recellularize various organs and tissues are largely different. Here we describe a method to effectively engineer a bioartificial colon by completely removing original cells from human intestinal tissues followed by repopulating the acellular tissue matrix with cell cultures. This method provides a novel approach for human intestinal regeneration and can be used to identify potential cancer driver genes.

Keywords: Decellularization; Disease modeling; Human intestine; Recellularization; Tissue engineering.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bioartificial Organs*
Cells, Cultured
Colon / cytology*
Colon / physiology*
Extracellular Matrix / metabolism*
Humans
Regenerative Medicine*
Tissue Engineering / methods*
Tissue Scaffolds*

Abstract

Publication types

MeSH terms

Grants and funding