Engineering of pectin-dopamine nano-conjugates for carrying ruthenium complex: A potential tool for biomedical applications

Jiajing Diao; Feng Bai; Ying Wang; Qianqian Han; Xi Xu; Hongmei Zhang; Qiang Luo; Yanqing Wang

doi:10.1016/j.jinorgbio.2018.11.016

Engineering of pectin-dopamine nano-conjugates for carrying ruthenium complex: A potential tool for biomedical applications

J Inorg Biochem. 2019 Feb:191:135-142. doi: 10.1016/j.jinorgbio.2018.11.016. Epub 2018 Nov 28.

Authors

Jiajing Diao¹, Feng Bai², Ying Wang², Qianqian Han², Xi Xu³, Hongmei Zhang⁴, Qiang Luo², Yanqing Wang⁵

Affiliations

¹ Institute of Environmental Toxicology and Environmental Ecology, Yancheng Teachers University, Yancheng City, Jiangsu Province 224007, People's Republic of China.
² Institute of Environmental Toxicology and Environmental Ecology, Yancheng Teachers University, Yancheng City, Jiangsu Province 224007, People's Republic of China; Chemistry and Chemical Engineering, Nanjing University of Technology, Nanjing City, Jiangsu Province 210009, People's Republic of China.
³ Center for Molecular Metabolism, Nanjing University of Science & Technology, B508, No. 364 Building, 200 Xiaolinwei Street, Nanjing 210094, People's Republic of China. Electronic address: xuxi@njust.edu.cn.
⁴ Institute of Environmental Toxicology and Environmental Ecology, Yancheng Teachers University, Yancheng City, Jiangsu Province 224007, People's Republic of China. Electronic address: hxzhm@126.com.
⁵ Institute of Environmental Toxicology and Environmental Ecology, Yancheng Teachers University, Yancheng City, Jiangsu Province 224007, People's Republic of China. Electronic address: wyqing76@126.com.

PMID: 30521965
DOI: 10.1016/j.jinorgbio.2018.11.016

Abstract

In this work, we designed polysaccharide-metal complex of dopamine-modified pectin linking ruthenium compound, which exhibited a certain inhibition specificity to human renal cell adenocarcinoma cell line 786-O. The chemical structure and physical properties of the polysaccharide-metal complex were well characterized by multiple analysis methods. The multi-spectral results revealed that pectin-dopamine have been successfully coordinated by ruthenium complex to form nano-conjugates, which self-assembled into relatively regular nanospheres of approximately 200 nm. The polysaccharide-metal complex has more amorphous and less viscosity than pectin, and cannot withstand as much strain as the pectin and pectin-dopamine systems. Pectin-dopamine can decrease the toxicity effect of Ru complex in normal cell line such as human normal renal epithelial cell line 293A.

Keywords: Anticancer activity; Dopamine; Nano-conjugates; Pectin; Ruthenium complex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocompatible Materials
Cell Line, Tumor
Coordination Complexes / chemistry*
Dopamine / chemistry*
Humans
Molecular Structure
Nanostructures*
Pectins / chemistry*
Ruthenium / chemistry*

Substances

Biocompatible Materials
Coordination Complexes
Ruthenium
Pectins
Dopamine