One method to establish Epstein-Barr virus-associated NK/T cell lymphoma mouse models

J Cell Mol Med. 2019 Feb;23(2):1509-1516. doi: 10.1111/jcmm.14057. Epub 2018 Nov 28.

Abstract

Novel nude mice model of human NK/T cell lymphoma were established by subcutaneously injecting two NK/T cell lymphoma cell lines into the right axillary region of mice and successful passages were completed by injecting cell suspension which was obtained through a 70-μm cell strainer. These mice models and corresponding cell clones have been successfully developed for more than 8 generations. The survival rates of both resuscitation and transplantation in NKYS and YT models were 90% and 70% correspondingly. Pathologically, the tumour cells in all passages of the lymphoma-bearing mice and cell lines obtained from tumours were parallel to initial cell lines. Immunologically, the tumour cells expressed the characteristics of the primary and essential NK/T lymphomas. The novel mice models maintained the essential features of human NK/T cell lymphoma, and they would be ideal tools in vivo for further research of human NK/T cell lymphoma.

Keywords: NK/T cell lymphoma; methods to establish mouse models; mouse models.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Disease Models, Animal*
  • Herpesvirus 4, Human / pathogenicity
  • Humans
  • Killer Cells, Natural / metabolism*
  • Killer Cells, Natural / pathology
  • Killer Cells, Natural / virology
  • Lymphoma, Extranodal NK-T-Cell / genetics
  • Lymphoma, Extranodal NK-T-Cell / pathology*
  • Lymphoma, Extranodal NK-T-Cell / virology
  • Mice
  • Mice, Nude