Fluorescent Wittig reagent as a novel ratiometric probe for the quantification of 5-formyluracil and its application in cell imaging

Chem Commun (Camb). 2018 Dec 4;54(97):13722-13725. doi: 10.1039/c8cc07541g.

Abstract

The chemically selective detection of natural nucleobase modifications has been regarded as the key step in understanding their important roles in epigenetics. Herein, for the first time, we introduce a Wittig reaction into the design of reaction-based fluorescent probes for ratiometrically detecting 5fU, selectively labelling 5fU-modified DNA and imaging intracellular 5fU produced by γ-irradiation.

MeSH terms

  • Cells / chemistry*
  • Cells / metabolism
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Molecular Structure
  • Optical Imaging*
  • Spectrometry, Fluorescence
  • Uracil / analogs & derivatives*
  • Uracil / analysis

Substances

  • Fluorescent Dyes
  • 5-formyluracil
  • Uracil