MicroRNA-107 Targets IKBKG and Sensitizes A549 Cells to Parthenolide

Anticancer Res. 2018 Nov;38(11):6309-6316. doi: 10.21873/anticanres.12987.

Abstract

Background/aim: Patients with advanced non-small cell lung cancer (NSCLC) frequently face a dismal prognosis because of lack of curative therapies. We, therefore, conducted a preclinical investigation of the therapeutic efficacy of microRNA-107 (miR-107).

Materials and methods: The effects of miR-107 on cell proliferation and target gene expression were studied. Combinatorial effects of miR-107 and parthenolide were evaluated.

Results: Cell proliferation was repressed in A549 NSCLC cells transfected with miR-107. Inhibitor of nuclear factor kappa B kinase subunit gamma was directly targeted by miR-107. Overexpression of miR-107 in A549 cells sensitized them to parthenolide along with a marked reduction of cyclin-dependent kinase 2.

Conclusion: Our findings unveil an important biological function of miR-107 in regulating lung cancer cell proliferation and elevating an antiproliferative effect of parthenolide on lung cancer cells, suggesting that miR-107 could be beneficial benefit treatment for advanced NSCLC.

Keywords: IKBKG; MicroRNA-107; NF-ĸB; combination effect; lung cancer; parthenolide.

MeSH terms

  • 3' Untranslated Regions
  • A549 Cells
  • Binding Sites
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Computational Biology
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • I-kappa B Kinase / chemistry
  • I-kappa B Kinase / genetics*
  • Lung Neoplasms / genetics*
  • MicroRNAs / genetics*
  • Sesquiterpenes / pharmacology*

Substances

  • 3' Untranslated Regions
  • IKBKG protein, human
  • MIRN107 microRNA, human
  • MicroRNAs
  • Sesquiterpenes
  • parthenolide
  • I-kappa B Kinase