The combined application of plant, microorganism, and amendment on the phytoremediation of heavy metals was optimized as a remediation technique for mine tailings by a field-scale orthogonal (L16) experiment, aimed to achieve the maximum of phytoremediation effect. Soybean, M. Circinelloides, and A3 amendment (organic fertilizer: rice husk: biochar: ceramsite = 1:1:2:1) were recommended as the best plant, microorganism, and amendment materials, respectively. With the combined plant, microorganism, amendment application, effective fractions of Cu, Zn, Pb, Cd, Mn were immobilized for decreased bioavailability, indicating the phytostabilization served as a major repair pathway. Plant length and biomass in the treatments were significantly higher than that in the control, indicating their phytoremediation potentials were enhanced. The final contents of heavy metals in soil were decreased, and the removal rates of soil heavy metals were in the order of Pb＞Cd＞Cu＞Zn＞Mn. Temporal variations of soil microorganism populations indicated that the abundance of soil microorganism in the treatments was significantly higher than that in the control, and bacteria became the dominant microbial species. Results showed that the soil organic matter and catalase, urease, phosphatase activities of the treatments were all significantly higher than that of the control. This study provided optimized combined plant, microorganism, amendment materials in the enhanced phytoremediation field to make up the deficiencies of the long-term phytoremediation for heavy metals.
Keywords: Amendment; Microorganism; Orthogonal experiment; Phytoremediation; Plant.
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