Molecular tools that block maturation of the nuclear lamin A and decelerate cancer cell migration

Bioorg Med Chem. 2018 Nov 1;26(20):5547-5554. doi: 10.1016/j.bmc.2018.10.001. Epub 2018 Oct 4.

Abstract

Lamin A contributes to the structure of nuclei in all mammalian cells and plays an important role in cell division and migration. Mature lamin A is derived from a farnesylated precursor protein, known as prelamin A, which undergoes post-translational cleavage catalyzed by the zinc metalloprotease STE24 (ZPMSTE24). Accumulation of farnesylated prelamin A in the nuclear envelope compromises cell division, impairs mitosis and induces an increased expression of inflammatory gene products. ZMPSTE24 has been proposed as a potential therapeutic target in oncology. A library of peptidomimetic compounds were synthesized and screened for their ability to induce accumulation of prelamin A in cancer cells and block cell migration in pancreatic ductal adenocarcinoma cells. The results of this study suggest that inhibitors of lamin A maturation may interfere with cell migration, the biological process required for cancer metastasis.

Keywords: Lamin A; Prelamin A; ZPMSTE24; Zinc metalloprotease STE24.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / drug therapy
  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / pharmacology*
  • Carcinoma, Pancreatic Ductal / drug therapy
  • Carcinoma, Pancreatic Ductal / metabolism
  • Carcinoma, Pancreatic Ductal / pathology
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Humans
  • Lamin Type A / metabolism*
  • Membrane Proteins / metabolism
  • Metalloendopeptidases / metabolism
  • Neoplasm Invasiveness / pathology
  • Neoplasm Invasiveness / prevention & control
  • Peptidomimetics / chemical synthesis
  • Peptidomimetics / chemistry*
  • Peptidomimetics / pharmacology*
  • Phosphinic Acids / chemical synthesis
  • Phosphinic Acids / chemistry
  • Phosphinic Acids / pharmacology

Substances

  • Antineoplastic Agents
  • Lamin Type A
  • Membrane Proteins
  • Peptidomimetics
  • Phosphinic Acids
  • prelamin A
  • Metalloendopeptidases
  • ZMPSTE24 protein, human

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