Molecular docking, and anti-biofilm activity of gold-complexed sulfonamides on Pseudomonas aeruginosa

Microb Pathog. 2018 Dec:125:393-400. doi: 10.1016/j.micpath.2018.10.004. Epub 2018 Oct 2.

Abstract

The antibacterial activity of sulfadiazine Au-PPh3, sulfadiazine Ph2P-Au-Au-PPh2, sulfamethoxazole Au-PPh3, sulfamethoxazole Ph2P-Au-Au-PPh2, sulfamethoxazole Au-PPh3 were tested against Pseudomonas aeruginosa. The antibacterial activity of sulfonamide was tested against P. aeruginosa through the MIC assay, quantitative analysis of biofilm inhibition and observation of biofilm formation with fluorescence microscopy. Besides, the compounds presented remarkable inhibition of P. aeruginosa biofilm formation. Furthermore, molecular docking was performed to identify the key structural features of these compounds with the binding site of the LasR receptor.

Keywords: Biofilm; Gold-complexed sulfonamides; LasR; PA01.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism
  • Biofilms / drug effects*
  • Gold / chemistry
  • Gold / pharmacology*
  • Microbial Sensitivity Tests
  • Microscopy, Fluorescence
  • Molecular Docking Simulation
  • Protein Binding
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / physiology
  • Sulfonamides / chemistry
  • Sulfonamides / pharmacology*
  • Trans-Activators / chemistry
  • Trans-Activators / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • LasR protein, Pseudomonas aeruginosa
  • Sulfonamides
  • Trans-Activators
  • Gold