Structural basis for brassinosteroid response by BIL1/BZR1

Shohei Nosaki; Takuya Miyakawa; Yuqun Xu; Akira Nakamura; Kei Hirabayashi; Tadao Asami; Takeshi Nakano; Masaru Tanokura

doi:10.1038/s41477-018-0255-1

Structural basis for brassinosteroid response by BIL1/BZR1

Nat Plants. 2018 Oct;4(10):771-776. doi: 10.1038/s41477-018-0255-1. Epub 2018 Oct 1.

Authors

Shohei Nosaki¹, Takuya Miyakawa¹, Yuqun Xu¹, Akira Nakamura¹, Kei Hirabayashi¹, Tadao Asami^{1

2}, Takeshi Nakano³, Masaru Tanokura⁴

Affiliations

¹ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
² Department of Biochemistry, King Abdulaziz University, Jeddah, Saudi Arabia.
³ Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Wako, Saitama, Japan.
⁴ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. amtanok@mail.ecc.u-tokyo.ac.jp.

PMID: 30287951
DOI: 10.1038/s41477-018-0255-1

Abstract

BRZ-INSENSITIVE-LONG HYPOCOTYL 1 (BIL1)/BRASSINAZOLE-RESISTANT 1 (BZR1) is a master transcription factor of brassinosteroid (BR) signalling. The varieties of nucleobase recognition of the NN-BRRE-core motif (NNCGTG), one of variant G-box motifs, distinguish BIL1/BZR1 from basic helix-loop-helix transcription factors, underlying the specific regulation of BR-responsive genes. Here, we show the non-canonical bHLH dimer formation of BIL1/BZR1 to optimize the interaction network with DNA and the orientation of a key residue for NN-BRRE-core motif recognition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / genetics
Arabidopsis / metabolism
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Basic Helix-Loop-Helix Transcription Factors / chemistry
Basic Helix-Loop-Helix Transcription Factors / genetics
Basic Helix-Loop-Helix Transcription Factors / metabolism*
Brassinosteroids / metabolism*
DNA, Plant / metabolism
DNA-Binding Proteins
Dimerization
Glycogen Synthase Kinase 3 / chemistry
Glycogen Synthase Kinase 3 / genetics
Glycogen Synthase Kinase 3 / metabolism*
Nuclear Proteins / chemistry
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Sequence Alignment

Substances

Arabidopsis Proteins
BZR1 protein, Arabidopsis
Basic Helix-Loop-Helix Transcription Factors
Brassinosteroids
DNA, Plant
DNA-Binding Proteins
Nuclear Proteins
AT2G30980 protein, Arabidopsis
Glycogen Synthase Kinase 3