Iridoviral infection can be reduced by UCHL1-loaded exosomes from the testis of Chinese giant salamanders (Andrias davidianus)

Abstract

Chinese giant salamander iridovirus (CGSIV) is a large double-stranded DNA virus that infects Chinese giant salamanders (CGSs) and is responsible for a high mortality rate of CGSs under certain conditions. It is generally believed that CGSIV is a horizontally transmitting virus that affects lower vertebrates. Exosomes from tissues and cells affect the mechanism of viral infections. UCHL1, a deubiquitinating enzyme, is indirectly involved in virus propagation via cytokine and chemokine suppression. In our study, a few CGSIVs were detected in the testis of the special symptom CGSs using PCR and immunofluorescence analysis. The exosomes originating in the testicular fluid was isolated and identified using the Nanosight NS300 system and scanning electron microscopy. The UCHL1-loaded exosomes may resist CGSIV entry by fusing with and remodeling CGSIV. UCHL1 in the primary testicular fibroblasts was maintained at a stable level to inhibit the infection and replication of CGSIV by secreting and sorting exosomes. These data provided a new insight into CGSIV being a type of horizontally transmitting virus.

Keywords: Chinese giant salamanders (Andrias davidianus); Exosome; Iridovirus; Testis; UCHL1.