Haploinsufficiency of Homeodomain Proteins Six3, Vax1, and Otx2 Causes Subfertility in Mice via Distinct Mechanisms

Neuroendocrinology. 2019;109(3):200-207. doi: 10.1159/000494086. Epub 2018 Sep 27.

Abstract

Haploinsufficiency occurs when loss of one copy of a diploid gene (hemizygosity) causes a phenotype. It is relatively rare, in that most genes can produce sufficient mRNA and protein from a single copy to prevent any loss of normal activity and function. Reproduction is a complex process relying on migration of GnRH neurons from the olfactory placode to the hypothalamus during development. We have studied 3 different homeodomain genes Otx2, Vax1, and Six3 and found that the deletion of one allele for any of these genes in mice produces subfertility or infertility in one or both sexes, despite the presence of one intact allele. All 3 heterozygous mice have reduced numbers of GnRH neurons, but the mechanisms of subfertility differ significantly. This review compares the subfertility phenotypes and their mechanisms.

Keywords: Heterozygous; Homeodomain; Infertility.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Eye Proteins / genetics*
  • Eye Proteins / metabolism
  • Gonadotropin-Releasing Hormone / metabolism
  • Haploinsufficiency*
  • Homeobox Protein SIX3
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism
  • Infertility / genetics*
  • Infertility / metabolism
  • Mice
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Neurons / metabolism
  • Neuropeptides / genetics*
  • Neuropeptides / metabolism
  • Otx Transcription Factors / genetics*
  • Otx Transcription Factors / metabolism
  • Phenotype

Substances

  • Eye Proteins
  • Homeodomain Proteins
  • Nerve Tissue Proteins
  • Neuropeptides
  • Otx Transcription Factors
  • Otx2 protein, mouse
  • Vax1 protein, mouse
  • Gonadotropin-Releasing Hormone