Infection perturbs Bach2- and Bach1-dependent erythroid lineage 'choice' to cause anemia

Nat Immunol. 2018 Oct;19(10):1059-1070. doi: 10.1038/s41590-018-0202-3. Epub 2018 Sep 24.

Abstract

Elucidation of how the differentiation of hematopoietic stem and progenitor cells (HSPCs) is reconfigured in response to the environment is critical for understanding the biology and disorder of hematopoiesis. Here we found that the transcription factors (TFs) Bach2 and Bach1 promoted erythropoiesis by regulating heme metabolism in committed erythroid cells to sustain erythroblast maturation and by reinforcing erythroid commitment at the erythro-myeloid bifurcation step. Bach TFs repressed expression of the gene encoding the transcription factor C/EBPβ, as well as that of its target genes encoding molecules important for myelopoiesis and inflammation; they achieved the latter by binding to their regulatory regions also bound by C/EBPβ. Lipopolysaccharide diminished the expression of Bach TFs in progenitor cells and promoted myeloid differentiation. Overexpression of Bach2 in HSPCs promoted erythroid development and inhibited myelopoiesis. Knockdown of BACH1 or BACH2 in human CD34+ HSPCs impaired erythroid differentiation in vitro. Thus, Bach TFs accelerate erythroid commitment by suppressing the myeloid program at steady state. Anemia of inflammation and myelodysplastic syndrome might involve reduced activity of Bach TFs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemia / etiology
  • Anemia / metabolism*
  • Animals
  • Basic-Leucine Zipper Transcription Factors / metabolism*
  • Cell Differentiation / physiology
  • Erythroid Cells / cytology
  • Erythroid Cells / metabolism
  • Erythropoiesis / physiology*
  • Humans
  • Infections / complications
  • Lipopolysaccharides / toxicity
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myelodysplastic Syndromes / etiology
  • Myelodysplastic Syndromes / metabolism

Substances

  • BACH1 protein, human
  • BACH2 protein, human
  • Bach1 protein, mouse
  • Bach2 protein, mouse
  • Basic-Leucine Zipper Transcription Factors
  • Lipopolysaccharides