AtROP6 is involved in reactive oxygen species signaling in response to iron-deficiency stress in Arabidopsis thaliana

Longmei Zhai; Chaohua Sun; Yi Feng; Duyue Li; Xiaofen Chai; Lei Wang; Qiran Sun; Guifen Zhang; Yi Li; Ting Wu; Xinzhong Zhang; Xuefeng Xu; Yi Wang; Zhenhai Han

doi:10.1002/1873-3468.13257

AtROP6 is involved in reactive oxygen species signaling in response to iron-deficiency stress in Arabidopsis thaliana

FEBS Lett. 2018 Oct;592(20):3446-3459. doi: 10.1002/1873-3468.13257. Epub 2018 Oct 8.

Authors

Longmei Zhai^{1

2}, Chaohua Sun^{1

2}, Yi Feng^{1

2}, Duyue Li^{1

2}, Xiaofen Chai^{1

2}, Lei Wang^{1

2}, Qiran Sun^{1

2}, Guifen Zhang^{1

2}, Yi Li³, Ting Wu^{1

2}, Xinzhong Zhang^{1

2}, Xuefeng Xu^{1

2}, Yi Wang^{1

2}, Zhenhai Han^{1

2}

Affiliations

¹ College of Horticulture, China Agricultural University, Beijing, China.
² Key Laboratory of Stress Physiology and Molecular Biology for Fruit Trees in Beijing Municipality, China Agricultural University, Beijing, China.
³ Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, USA.

PMID: 30238451
DOI: 10.1002/1873-3468.13257

Abstract

Understanding the mechanism of iron (Fe)-deficiency responses is crucial for improving plant Fe bioavailability. Here, we found that the Arabidopsis Rho-like GTPase 6 mutant (rop6) is less sensitive to Fe-deficiency responses and has reduced levels of reactive oxygen species (ROS) compared to wild-type (WT), while AtROP6-overexpressing seedlings exhibit more sensitivity to Fe-deficiency responses and has higher levels of ROS compared to WT. Moreover, treatment with H₂ O₂ improves the sensitivity to Fe-deficiency responses in rop6 mutants. By using the yeast two-hybrid system, we further demonstrate the direct interaction between AtROP6 and Arabidopsis respiratory burst oxidase homolog D (AtRBOHD), which controls the generation of ROS. Overall, we suggest that AtROP6 is involved in AtRBOHD-mediated ROS signaling to modulate Fe-deficiency responses in Arabidopsis thaliana.

Keywords: AtROP6; iron deficiency; reactive oxygen species.

MeSH terms

Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Gene Expression Regulation, Plant
Hydrogen Peroxide / pharmacology
Iron / metabolism*
Monomeric GTP-Binding Proteins / genetics
Monomeric GTP-Binding Proteins / metabolism*
Mutation
NADPH Oxidases / genetics
NADPH Oxidases / metabolism
Oxidants / pharmacology
Plants, Genetically Modified
Protein Binding
Reactive Oxygen Species / metabolism*
Seedlings / genetics
Seedlings / metabolism
Signal Transduction*
Stress, Physiological / drug effects

Substances

Arabidopsis Proteins
Oxidants
ROP6 protein, Arabidopsis
Reactive Oxygen Species
Hydrogen Peroxide
Iron
respiratory burst oxidase homolog D, Arabidopsis
NADPH Oxidases
Monomeric GTP-Binding Proteins