Structure and function of the repressor of bacteriophage lambda. III. Molecular cloning of the high-affinity mutant cI gene of lambda and studies of the properties of the clones

Mol Gen Genet. 1986 Sep;204(3):540-2. doi: 10.1007/BF00331037.

Abstract

The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively, the level of repressor in a monolysogen of lambda cIha. Results of the study of certain properties of the bacteria carrying these plasmids suggest that the ha repressor also has a higher affinity for the virulent mutant operators as well as the prm promoter of lambda.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda / genetics*
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Escherichia coli / genetics*
  • Genes, Viral*
  • Mutation*
  • Plasmids
  • Repressor Proteins / genetics*
  • Transcription Factors / genetics*

Substances

  • Repressor Proteins
  • Transcription Factors
  • DNA Restriction Enzymes